Inbred corn line G06-NP2899

ABSTRACT

Basically, this invention provides for an inbred corn line designated G06-NP2899, methods for producing a corn plant by crossing plants of the inbred line G06-NP2899 with plants of another corn plants. The invention relates to the various parts of inbred G06-NP2899 including culturable cells. This invention also relates to methods for introducing transgenic transgenes into inbred corn line G06-NP2899 and plants produced by said methods.

This application is claiming priority from the U.S. application filed onFeb. 28, 2006, application Ser. No. 11/364,837.

FIELD OF THE INVENTION

This invention is in the field of corn breeding, specifically relatingto an inbred corn line designated G06-NP2899. This invention also is inthe field of hybrid maize production employing the present inbred.

BACKGROUND OF THE INVENTION

The original maize plant was indigenous to the Western Hemisphere. Theplants were weedlike and only through the efforts of early breeders werecultivated crop species developed. The crop cultivated by earlybreeders, like the crop today, could be wind pollinated. The physicaltraits of maize are such that wind pollination results inself-pollination or cross-pollination between plants. Each maize planthas a separate male and female flower that contributes to pollination,the tassel and ear, respectively. Natural pollination occurs when windtransfers pollen from tassel to the silks on the corn ears. This type ofpollination has contributed to the wide variation of maize varietiespresent in the Western Hemisphere.

The development of a planned breeding program for maize only occurred inthe last century. A large part of the development of the maize productinto a profitable agricultural crop was due to the work done by landgrant colleges. Originally, maize was an open pollinated variety havingheterogeneous genotypes. The maize farmer selected uniform ears from theyield of these genotypes and preserved them for planting the nextseason. The result was a field of maize plants that were segregating fora variety of traits. This type of maize selection led to, at most,incremental increases in seed yield.

Large increases in seed yield were due to the work done by land grantcolleges that resulted in the development of numerous hybrid cornvarieties in planned breeding programs. Hybrids were developed frominbreds which were developed by selecting corn lines and selfing theselines for several generations to develop homozygous pure inbred lines.One selected inbred line was emasculated and another selected inbredline pollinated the emasculated inbred to produce hybrid seed F1 on theemasculated inbred line. Emasculation of the inbred usually is done bydetasseling the seed parent; however, emasculation can be done in anumber of ways. For example an inbred could have a male sterility factorwhich would eliminate the need to detassel the inbred.

In the early seventies the hybrid corn industry attempted to introduceCMS (cytoplasmic male sterility) into a number of inbred lines.Unfortunately, the CMS inbreds also introduced some very poor agronomicperformance traits into the hybrid seed which caused farmers concerncausing the maize industry to shy away from CMS material for a couple ofdecades thereafter.

However, in the last 10-15 years a number of different male sterilitysystems for maize have been successfully deployed. The mosttraditionally of these male sterility and/or CMS systems for maizeparallel the CMS type systems that have been routinely used in hybridproduction in sunflower.

In the standard CMS system there are three different maize linesrequired to make the hybrid. First, there is a cytoplasmic male-sterileline usually carrying the CMS or some other form of male sterility. Thisline will be the seed producing parent line. Second, there must be afertile inbred line that is the same or isogenic with the seed producinginbred parent but lacking the trait of male sterility. This is amaintainer line needed to make new inbred seed of the seed producingmale sterile parent. Third there is a different inbred which is fertile,has normal cytoplasm and carries a fertility restoring gene. This lineis called the restorer line in the CMS system. The CMS cytoplasm isinherited from the maternal parent (or the seed producing plant),therefore for the hybrid seed produced on such plant to be fertile thepollen used to fertilize this plant must carry the restorer gene. Thepositive aspect of this is that it allows hybrid seed to be producedwithout the need for detasseling the seed parent. However, this systemdoes require breeding of all three types of lines: 1) male sterile—tocarry the CMS: 2) the maintainer line; and, 3) the line carrying thefertility restorer gene.

In some instances, sterile hybrids are produced and the pollen necessaryfor the formation of grain on these hybrids is supplied by interplantingof fertile inbreds in the field with the sterile hybrids.

Whether the seed producing plant is emasculated by detasseling or by CMSor by transgenes, the seed produced by crossing two inbreds in thismanner is hybrid seed. This hybrid seed is F1 hybrid seed. The grainproduced by a plant grown from a F1 hybrid seed is referred to as F2 orgrain. Although, all F1 seed and plants, produced by this hybrid seedproduction system using the same two inbreds should be substantially thesame, all F2 grain produced from the F1 plant will be segregating maizematerial.

The hybrid seed production produces hybrid seed which is heterozygous.The heterozygosis results in hybrid plants, which are robust andvigorous plants. Inbreds on the other hand are mostly homozygous. Thishomozygosity renders the inbred lines less vigorous. Inbred seed can bedifficult to produce since the inbreeding process in corn linesdecreases the vigor. However, when two inbred lines are crossed, thehybrid plant evidences greatly increased vigor and seed yield comparedto open pollinated, segregating maize plants. An important consequenceof the homozygosity and the homogenity of the inbred maize lines is thatall hybrid seed produced from any cross of two such elite lines will bethe same hybrid seed and make the same hybrid plant. Thus the use ofinbreds makes hybrid seed which can be reproduced readily.

The ultimate objective of the commercial maize seed companies is toproduce high yielding, agronomically sound plants that perform well incertain regions or areas of the Corn Belt. To produce these types ofhybrids, the companies must develop inbreds, which carry needed traitsinto the hybrid combination. Hybrids are not often uniformly adapted forthe entire Corn Belt, but most often are specifically adapted forregions of the Corn Belt. Northern regions of the Corn Belt requireshorter season hybrids than do southern regions of the Corn Belt.Hybrids that grow well in Colorado and Nebraska soils may not flourishin richer Illinois and Iowa soil. Thus, a variety of major agronomictraits is important in hybrid combination for the various Corn Beltregions, and has an impact on hybrid performance.

Inbred line development and hybrid testing have been emphasized in thepast half-century in commercial maize production as a means to increasehybrid performance. Inbred development is usually done by pedigreeselection. Pedigree selection can be selection in an F₂ populationproduced from a planned cross of two genotypes (often elite inbredlines), or selection of progeny of synthetic varieties, open pollinated,composite, or backcrossed populations. This type of selection iseffective for highly inheritable traits, but other traits, for example,yield requires replicated test crosses at a variety of stages foraccurate selection.

Maize breeders select for a variety of traits in inbreds that impacthybrid performance along with selecting for acceptable parental traits.Such traits include: yield potential in hybrid combination; dry down;maturity; grain moisture at harvest; greensnap; resistance to rootlodging; resistance to stalk lodging; grain quality; disease and insectresistance; ear and plant height. Additionally, Hybrid performance willdiffer in different soil types such as low levels of organic matter,clay, sand, black, high pH, low pH; or in different environments such aswet environments, drought environments, and no tillage conditions. Thesetraits appear to be governed by a complex genetic system that makesselection and breeding of an inbred line extremely difficult. Even if aninbred in hybrid combination has excellent yield (a desiredcharacteristic), it may not be useful because it fails to haveacceptable parental traits such as seed yield, seed size, pollenproduction, good silks, plant height, etc.

To illustrate the difficulty of breeding and developing inbred lines,the following example is given. Two inbreds compared for similarity of29 traits differed significantly for 18 traits between the two lines. If18 simply inherited single gene traits were polymorphic with genefrequencies of 0.5 in the parental lines, and assuming independentsegregation (as would essentially be the case if each trait resided on adifferent chromosome arm), then the specific combination of these traitsas embodied in an inbred would only be expected to become fixed at arate of one in 262,144 possible homozygous genetic combinations.Selection of the specific inbred combination is also influenced by thespecific selection environment on many of these 18 traits which makesthe probability of obtaining this one inbred even more remote. Inaddition, most traits in the corn genome are regrettably not singledominant genes but are multi-genetic with additive gene action notdominant gene action. Thus, the general procedure of producing a nonsegregating F₁ generation and self pollinating to produce a F₂generation that segregates for traits and selecting progeny with thevisual traits desired does not easily lead to a useful inbred. Greatcare and breeder expertise must be used in selection of breedingmaterial to continue to increase yield and the agronomics of inbreds andresultant commercial hybrids.

Certain regions of the Corn Belt have specific difficulties that otherregions may not have. Thus the hybrids developed from the inbreds haveto have traits that overcome or at least minimize these regional growingproblems. Examples of these problems include in the eastern corn beltGray Leaf Spot, in the north cool temperatures during seedlingemergence, in the Nebraska region CLN (corn Lethal necrosis and in thewest soil that has excessively high pH levels. The industry oftentargets inbreds that address these issues specifically forming nicheproducts. However, the aim of most large seed producers is to provide anumber of traits to each inbred so that the corresponding hybrid canuseful in a broader regions of the Corn Belt. The new biotechnologytechniques such as Microsatellites, RFLPs, RAPDs and the like haveprovided breeders with additional tools to accomplish these goals.

SUMMARY OF THE INVENTION

The present invention relates to an inbred corn line G06-NP2899.Specifically, this invention relates to plants and seeds of this line.Additionally, this relates to a method of producing from this inbred,hybrid seed corn and hybrid plants with seeds from such hybrid seed.More particularly, this invention relates to the unique combination oftraits that combine in corn line G06-NP2899.

Generally then, broadly the present invention includes an inbred cornseed designated G06-NP2899. This seed produces a corn plant.

The invention also includes the tissue culture of regenerable cells ofG06-NP2899 wherein the cells of the tissue culture regenerates plantscapable of expressing the genotype of G06-NP2899. The tissue culture isselected from the group consisting of leaf, pollen, embryo, root, roottip, guard cell, ovule, seed, anther, silk, flower, kernel, ear, cob,husk and stalk, cell and protoplast thereof. The corn plant regeneratedfrom G06-NP2899 or any part thereof is included in the presentinvention. The present invention includes regenerated corn plants thatare capable of expressing G06-NP2899's genotype, phenotype or mutants orvariants thereof.

The invention extends to hybrid seed produced by planting, inpollinating proximity which includes using preserved maize pollen asexplained in U.S. Pat. No. 5,596,838 to Greaves, seeds of corn inbredlines G06-NP2899 and another inbred line if preserved pollen is notused; cultivating corn plants resulting from said planting; preventingpollen production by the plants of one of the inbred lines if two areemployed; allowing cross pollination to occur between said inbred lines;and harvesting seeds produced on plants of the selected inbred. Thehybrid seed produced by hybrid combination of plants of inbred corn seeddesignated G06-NP2899 and plants of another inbred line are apart of thepresent invention. This inventions scope covers hybrid plants and theplant parts including the grain and pollen grown from this hybrid seed.

The invention further includes a method of hybrid F1 production. A firstgeneration (F1) hybrid corn plant produced by the process of plantingseeds of corn inbred line G06-NP2899; cultivating corn plants resultingfrom said planting; permitting pollen from another inbred line to crosspollinate inbred line G06-NP2899; harvesting seeds produced on plants ofthe inbred; and growing a harvested seed are part of the method of thisinvention.

The present invention also encompasses a method of introducing at leastone targeted trait into maize inbred line comprising the steps of: (A)crossing plant grown from the present invention seed which is therecurrent parent, representative seed of which has been deposited, withthe donor plant of another maize line that comprises at least one targettrait selected from the group consisting of male sterility, herbicideresistance, insect resistance, disease resistance, amylose starch, andwaxy starch to produce F1 plants; (b) selecting from the F1 plants thathave at least one of the targeted trait, forming a pool of progenyplants with the targeted trait; (c) crossing the pool of progeny plantswith the present invention which is the recurrent parent to producebackcrossed progeny plants with the targeted trait; (d) selecting forbackcrossed progeny plants that have at least one of the target traitand physiological and morphological characteristics of maize inbred lineof the recurrent parent, listed in Table 1 forming a pool of selectedbackcrossed progeny plants; and (e) crossing the selected backcrossedprogeny plants to the recurrent parent and selecting from the resultingplants for the targeted trait and physiological and morphologicalcharacteristics of maize inbred line of the recurrent parent, listed inTable 1 and reselecting from the pool of resulting plants and repeatingthe crossing to the recurrent parent and selecting step in succession toform a plant that comprise the desired trait and all of thephysiological and morphological characteristics of maize inbred line ofthe recurrent parent if the present invention listed in Table 1 asdetermined at the 5% significance level when grown in the sameenvironmental conditions.

This method and the following method of introducing traits can be donewith less back crossing events if the trait and/or the genotype of thepresent invention are selected for or identified through the use ofmarkers. SSR, microsatellites, SNP and the like decrease the amount ofbreeding time required to locate a line with the desired trait or traitsand the characteristics of the present invention. Backcrossing in two oreven three traits (for example the glyphosate, Europe corn borer, cornrootworm resistant genes) is routinely done with the use of markerassisted breeding techniques. This introduction of transgenes ormutations into a maize line is often called single gene conversion.Although, presently more than one gene particularly transgenes ormutations which are readily tracked with markers can be moved during thesame “single gene conversion” process, resulting in a line with theaddition of more targeted traits than just the one, but still having thecharacteristics of the present invention plus those characteristicsadded by the targeted traits.

The method of introducing a desired trait into maize inbred linecomprising: (a) crossing plant grown from the present invention seed,representative seed of which has been deposited the recurrent parent,with plant of another maize line that comprises at least one targettrait selected from the group consisting of nucleic acid encoding anenzyme selected from the group consisting of phytase, stearyl-ACPdesaturase, fructosyltransferase, levansucrase, amylase, invertase andstarch branching enzyme, the donor parent to produce F1 plants; (b)selecting for the targeted trait from the F1 plants, forming a pool ofprogeny plants; (c) crossing the progeny plants with the recurrentparent to produce backcrossed progeny plants; (d) selecting forbackcrossed progeny plants that have at least one of the target traitand physiological and morphological characteristics of maize inbred lineof the present invention a listed in Table 1 forming a pool ofbackcrossed progeny plants; and repeating a step of crossing the newpool with the recurrent parent and selecting for the targeted trait andthe recurrent parents characteristics until the selected plant isessentially the recurrent parent with the targeted trait or targetedtraits. This selection and crossing may take at least 4 backcrosses ifmarker assisted breeding is not employed.

The inbred line and seed of the present invention are employed to carrythe agronomic package into the hybrid. Additionally, the inbred line isoften carrying transgenes that are introduced in to the hybrid seed.

Likewise included is a first generation (F1) hybrid corn plant producedby the process of planting seeds of corn inbred line G06-NP2899;cultivating corn plants resulting from said planting; permitting pollenfrom inbred line G06-NP2899 to cross pollinate another inbred line;harvesting seeds produced on plants of the inbred; and growing a plantfrom such a harvested seed.

A number of different techniques exist which are designed to avoiddetasseling in maize hybrid production. Some examples are switchablemale sterility, lethal genes in the pollen or anther, inducible malesterility, male sterility genes with chemical restorers. There arenumerous patented means of improving upon the hybrid production system.Some examples include U.S. Pat. No. 6,025,546, which relates to the useof tapetum-specific promoters and the barnase gene to produce malesterility; U.S. Pat. No. 6,627,799 relates to modifying stamen cells toprovide male sterility. Therefore, one aspect of the current inventionconcerns the present invention comprising one or more gene(s) capable ofrestoring male fertility to male-sterile maize inbreds or hybrids and/orgenes or traits to produce male sterility in maize inbreds or hybrids.

The inbred corn line G06-NP2899 and at least one transgenic gene adaptedto give G06-NP2899 additional and/or altered phenotypic traits arewithin the scope of the invention. Such transgenes are usuallyassociated with regulatory elements (promoters, enhancers, terminatorsand the like). Presently, transgenes provide the invention with traitssuch as insect resistance, herbicide resistance, disease resistanceincreased or deceased starch or sugars or oils, increased or decreasedlife cycle or other altered trait.

The present invention includes inbred corn line G06-NP2899 and at leastone transgenic gene adapted to give G06-NP2899 modified starch traits.Furthermore this invention includes the inbred corn line G06-NP2899 andat least one mutant gene adapted to give modified starch, acid or oiltraits, i.e. amylase, waxy, amylose extender or amylose. The presentinvention includes the inbred corn line G06-NP2899 and at least onetransgenic gene: bacillus thuringiensis, the bar or pat gene encodingPhosphinothricin acetyl Transferase, Gdha gene, GOX, VIP, EPSP synthasegene, low phytic acid producing gene, and zein. The inbred corn lineG06-NP2899 and at least one transgenic gene useful as a selectablemarker or a screenable marker is covered by the present invention.

A tissue culture of the regenerable cells of hybrid plants produced withuse of G06-NP2899 genetic material is covered by this invention. Atissue culture of the regenerable cells of the corn plant produced bythe method described above is also included.

DEFINITIONS

In the description and examples, which follow, a number of terms areused. In order to provide a clear and consistent understanding of thespecifications and claims, including the scope to be given such terms,the following definitions are provided.

PLANT

This term includes the entire plant and its plant cells, plantprotoplasts made from its cells, plant cell tissue cultures from whichcorn plants can be regenerated, plant calli, plant clumps, and plantcells that are intact in plants or parts of plants, such as embryos,pollen, flowers, kernels, ears, cobs, leaves, husks, stalks, roots, roottips, anthers, silk and the like, and this term also includes anymutated gene, transgenic DNA or (RNA) or portion thereof that have beenintroduced into the plant by whatever method.TWTThe measure of the weight of grain in pounds for a one bushel volumeadjusted for percent grain moisture.% DROPPED EARS (DE) Or PCTDEThe number of plants per plot, which dropped their primary ear, dividedby the total number of plants per plot.% ROOT LODGE (RL) Or PCTRLPercentage of plants per plot leaning more that 30 degrees from verticaldivided by total plants per plot.YIELD (YLD)Actual yield of grain at harvest adjusted to 15.5% moisture.Measurements are reported in bushels per acre.MOISTUREThe average percentage grain moisture of an inbred or hybrid at harvesttime.% STALK LODGE (SL) Or PCTSLPercentage of plants per plot with the stalk broken below the primaryear node divided by the total plants per plot.GREEN SNAP (Gsnap): Count the number of plants in yield rows thatsnapped below the ear due to brittleness associated with high winds. ForFET plots, count snapped plants out of 50 from two locations in eachhybrid strip, sum, and record the percentage.STAY-GREEN (Sgreen): This is an assessment of the ability of a grainhybrid to retain green color as maturity approaches (taken near the timeof black-layer) and should not be a reflection of hybrid maturity orleaf disease. Record % of green tissue.STAND: Shall mean the number of plants in the plot that were harvested.

Color Choices: 1. light green 10. pink-orange 19. white 2. medium green11. pink 20. white capped 3. dark green 12. light red 21. buff 4. verydark 13. cherry red 22. tan  green 14. red 23. brown 5. green-yellow 15.red and white 24. bronze 6. pale yellow 16. pale purple 25. variegated7. yellow   (describe) 26. other (describe) 8. yellow-orange 17. purple9. salmon 18. colorless Form Input # ABR. Description Value A1 EMRGNFinal number of plants per plot # A2 REGNN Region Developed: 1.Northwest # 2. Northcentral 3. Northeast 4. Southeast 5. Southcentral 6.Southwest 7. Other A3 CRTYN Cross type: 1. sc 2. dc 3. 3w 4. msc # 5.m3w 6. inbred 7. rel. line 8. other A4 KRTPN Kernel type: 1. sweet 2.dent 3. flint # 4. flour 5. pop 6. ornamental 7. pipecorn 8. other A5EMERN Days to Emergence EMERN #Days B1 ERTLP % Root lodging: (beforeanthesis): #% B2 GRSNP % Brittle snapping: (before anthesis): #% C1TBANN Tassel branch angle of 2nd primary lateral degree branch (atanthesis): C10 HUPSN Heat units to 50% pollen shed: (from #HU emergence)C11 SLKCN Silk color: #/Munsell value C12 HU5SN Heat units to 50% silk:(from emergence) #HU C13 DSAZN Days to 50% silk in adapted zone: #DaysC14 HU9PN Heat units to 90% pollen shed: (from #HU emergence) C15 HU19NHeat units from 10% to 90% pollen shed: #HU C16 DA19N Days from 10% to90% pollen shed: #Days C2 LSPUR Leaf sheath pubescence of second leaf #above the ear (at anthesis) 1-9 (1 = none): C3 ANGBN Angle between stalkand 2nd leaf degree above the ear (at anthesis): C4 CR2LN Color of 2ndleaf above the ear #/Munsell (at anthesis): value C5 GLCRN Glume Color:#/Munsell value C6 GLCBN Glume color bars perpendicular to their # veins(glume bands): 1. absent 2. present C7 ANTCN Anther color: #/Munsellvalue C8 PLQUR Pollen Shed: 1-9 (0 = male sterile) # C9 HU1PN Heat unitsto 10% pollen shed: (from #HU emergence) D1 LAERN Number of leaves abovethe top ear node: # D10 LTBRN Number of lateral tassel branches that #originate from the central spike: D11 EARPN Number of ears per stalk: #D12 APBRR Anthocyanin pigment of brace roots: # 1. absent 2. faint 3.moderate 4. dark D13 TILLN Number of tillers: # D14 HSKCN Husk color 25days after 50% silk: (fresh) #/Munsell value D2 MLWVR Leaf marginalwaves: 1-9 (1 = none) # D3 LFLCR Leaf longitudinal creases: 1-9 (1 =none) # D4 ERLLN Length of ear leaf at the top ear node: #cm D5 ERLWNWidth of ear leaf at the top ear node at the #cm widest point: D6 PLHTNPlant height to tassel tip: #cm D7 ERHCN Plant height to the top earnode: #cm D8 LTEIN Length of the internode between the ear #cm node andthe node above: D9 LTASN Length of the tassel from top leaf collar #cmto tassel tip: E1 HSKDN Husk color 65 days after 50% silk: (dry)#/Munsell value E10 DSGMN Days from 50% silk to 25% grain moisture #Daysin adapted zone: E11 SHLNN Shank length: #cm E12 ERLNN Ear length: #cmE13 ERDIN Diameter of the ear at the midpoint: #mm E14 EWGTN Weight of ahusked ear: #gm E15 KRRWR Kernel rows: 1. indistinct 2. distinct # E16KRNAR Kernel row alignment: 1. straight # 2. slightly curved 3. curvedE17 ETAPR Ear taper: 1. slight 2. average 3. extreme # E18 KRRWN Numberof kernel rows: # E19 COBCN Cob color: #/Munsell value E2 HSKTR Husktightness 65 days after 50% silk: 1-9 # (1 = loose) E20 COBDN Diameterof the cob at the midpoint: #mm E21 YBUAN Yield: #kg/ha E22 KRTENEndosperm type: 1. sweet 2. extra sweet 3 3. normal 4. high amylose 5.waxy 6. high protein 7. high lysine 8. super sweet 9. high oil 10. otherE23 KRCLN Hard endosperm color: #/Munsell value E24 ALECN Aleuronecolor: #/Munsell value E25 ALCPR Aleurone color pattern: 1. homozygous #2. segregating E26 KRLNN Kernel length: #mm E27 KRWDN Kernel width: #mmE28 KRDPN Kernel thickness: #mm E29 K1KHN 100 kernel weight: #gm E3HSKCR Husk extension: 1. short (ear exposed) # 2. medium (8 cm) 3. long(8-10 cm) 4. very long (>10 cm) E30 KRPRN % round kernels on 13/64slotted screen: #% E4 HEPSR Position of ear 65 days after 50% silk: # 1.upright 2. horizontal 3. pendent E5 STGRP Staygreen 65 days afteranthesis: 1-9 # (1 = worst) E6 DPOPP % dropped ears 65 days afteranthesis: % E7 LRTRP % root lodging 65 days after anthesis: % E8 HU25NHeat units to 25% grain moisture: (from #HU emergence) E9 HUSGN Heatunits from 50% silk to 25% grain #HU moisture in adapted zone:

DETAILED DESCRIPTION OF THE INVENTION

G06-NP2899 is shown in comparison with a number of standard inbreds usedfor comparison by the US PVP office. The present inbred is in thehybrid, X72274 and it forms a 111 day hybrid with RM of 7.2.

The inbred provides uniformity and stability within the limits ofenvironmental influence for traits as described in the VarietyDescription Information (Table 1) that follows.

The inbred has been self-pollinated for a sufficient number ofgenerations to give inbred uniformity. During plant selection in eachgeneration, the uniformity of plant type was selected to ensurehomozygosity and phenotypic stability. The line has been increased inisolated farmland environments with data on uniformity and agronomictraits being observed to assure uniformity and stability. No varianttraits have been observed or are expected in G06-NP2899.

The best method of producing the invention, G06-NP2899 which issubstantially homozygous, is by planting the seed of G06-NP2899 which issubstantially homozygous and self-pollinating or sib pollinating theresultant plant in an isolated environment, and harvesting the resultantseed.

TABLE 1 G06-NP2899 VARIETY DESCRIPTION INFORMATION  Trait  B37  B64  B68 B73  B76

 H84  H89  MS71  YGSMN  39.4  37.2  53.4  88.9  55.1

 74.7  47.3  54.9  LRTLP  0.0  0.0  0.0  0.0  0.0

 0.0  0.0  0.0  ERTLP  0.0  0.0  0.0  0.0  0.0

 0.0  0.0  0.0  NHL_P  0.0  0.0  0.0  0.0  0.0

 0.0  0.0  0.0  EMRGN  57.8  52.0  58.5  62.5  57.5

 37.0  56.5  54.8  STD_N  57.8  52.0  58.5  62.5  57.5

 37.0  56.5  54.8  GRSNP  −0.1  0.0  0.0  0.0  0.0

 0.0  0.0  −0.1  HSKCR  2.0  2.5  3.0  3.0  1.5

 2.0  2.0  2.0  HU5SN  776.5  1438.0  1527.0  1341.0  1332.5

 1401.5  1289.0  693.5  PLHTN  225.8  236.9  219.8  227.9  191.4

 195.2  143.8  164.0  ERHTN  95.6  106.6  84.0  91.8  80.1

 64.3  35.8  60.0  HU1PN  670.5  1364.5  1355.0  1241.0  1279.0

 1242.0  1177.0  631.0  HU9PN  723.5  1491.0  1447.0  1341.0  1371.5

 1342.0  1327.0  693.5  HUPSN  693.5  1424.5  1405.5  1315.0  1323.5

 1295.5  1250.0  656.5  PLQUR  7.0  6.0  7.0  7.5  6.5

 6.5  6.5  9.0  EARPN  1.2  1.5  1.2  1.0  1.0

 1.4  1.2  1.2  SHLNN  9.0  10.8  12.8  8.3  8.0

 14.8  5.5  8.0  ALCPR  1.0  1.0  1.0  1.0  1.0

 1.0  1.0  1.0  ALECN  18.0  18.0  18.0  18.0  18.0

 18.0  18.0  18.0  ANTCN  14.0  3.5  5.5  6.5  14.0

 13.0  5.0  5.0  COBCN  11.0  19.0  13.5  14.0  11.0

 14.0  19.0  14.0  CR2LN  4.0  4.0  4.0  4.0  4.0

 4.0  4.0  3.0  CRTYN  6.0  6.0  6.0  6.0  6.0

 6.0  6.0  6.0  DA19N  2.5  5.5  4.5  4.0  4.0

 4.0  7.0  2.5  DSAZN  32.5  60.5  64.0  56.0  56.0

 59.0  54.0  29.0  DSGMN  47.0  51.0  49.0  53.0  54.5

 54.0  50.0  43.0  GLCBN  1.0  2.0  1.0  1.0  1.0

 1.0  2.0  1.0  GLCRN  2.0  2.5  1.5  2.0  2.0

 3.0  2.0  2.0  HSKCN  2.0  2.5  2.0  2.0  2.0

 3.0  3.0  2.0  HSKDN  21.0  21.0  21.0  21.0  21.0

 21.0  21.0  21.0  HU19N  53.0  126.5  92.0  100.0  92.5

 100.0  150.0  62.5  HU25N  2545.0  2522.5  2554.0  2489.5  2501.0

 2557.5  2387.0  2335.0  HUSGN  992.0  1084.5  1027.0  1148.5  1168.5

 1156.0  1098.0  948.0  KRCLN  8.0  8.0  8.0  8.0  8.0

 8.0  8.0  8.0  KRTEN  3.0  3.0  3.0  3.0  3.0

 3.0  3.0  3.0  KRTPN  2.0  2.0  2.0  2.0  2.0

 2.0  2.0  2.0  SLKCN  5.0  13.0  1.0  3.0  3.0

 1.0  3.0  5.0  LTEIN  14.0  14.0  14.5  17.0  13.0

 12.0  11.0  11.0  ERLWN  7.8  7.9  7.7  8.2  9.6

 7.9  7.3  6.4  ERLLN  72.8  92.0  91.5  75.3  67.4

 72.7  61.2  71.5  LAERN  6.6  6.3  6.9  6.0  5.7

 7.6  7.2  6.0  ANGBN  20.0  27.5  40.0  20.0  25.0

 27.5  45.0  30.0  LTBRN  8.8  7.9  8.1  6.6  6.2

 5.1  3.3  12.0  TBANN  35.0  45.0  37.5  15.0  40.0

 37.5  40.0  30.0  LTASN  37.7  46.8  38.1  40.6  37.5

 31.8  32.4  36.8  ERLNN  14.5  18.1  17.4  14.6  13.5

 14.2  13.8  14.2  ERDIN  36.4  36.0  38.3  44.7  40.1

 41.2  34.6  34.8  EWGTN  62.4  66.3  76.4  112.8  72.8

 98.6  65.8  75.6  KRRWN  12.0  13.0  15.2  17.6  13.2

 14.8  11.3  15.2  KRLNN  8.5  9.0  10.0  10.8  10.8

 11.5  8.8  9.0  KRWDN  8.0  7.5  7.0  7.3  9.5

 8.3  8.3  7.0  KRDPN  5.5  5.0  4.8  5.0  5.5

 5.3  4.5  4.5  KRPRN  97.0  81.0  61.0  40.0  84.0

 57.0  46.5  50.0  COBDN  22.7  24.2  25.5  27.8  26.0

 24.3  22.2  22.9  APBRR  2.0  3.0  4.0  3.5  2.5

 2.0  1.5  2.0  LSPUR  1.0  7.0  7.5  5.0  3.5

 2.0  2.0  4.0  MLWVR  8.0  6.0  4.5  5.0  6.5

 7.0  3.5  3.0  LFLCR  3.0  3.0  4.0  3.5  4.5

 2.5  4.0  3.0  HSKTR  7.0  7.5  6.5  6.5  3.0

 4.5  5.5  4.0  HEPSR  1.0  1.0  1.0  2.0  1.0

 1.0  3.0  3.0  KRRWR  1.0  1.5  2.0  2.0  2.0

 2.0  2.0  2.0  ETAPR  2.0  2.0  2.0  1.5  1.0

 1.0  1.0  2.0  K1KHN  29.0  29.5  24.0  25.5  30.0

 29.0  23.0  20.0  TILLN  0.0  0.0  0.0  0.0  0.0

 0.0  0.0  0.0  TILLP  0.0  0.0  0.0  0.0  0.0

 0.0  0.0  0.0  DROPP  0.0  0.0  0.0  0.0  0.0

 0.0  3.6  0.0  KERAR  3.0  2.0  1.0  1.0  1.5

 2.0  1.5  1.0 Trait Mo17 N192 NC268 Pa91 Va26 Va35 W64a YGSMN 87.0 62.250.3 78.9 76.0 100.3 70.6 LRTLP 0.0 0.0 0.0 0.0 0.0 0.0 0.0 ERTLP 0.00.0 0.0 0.0 0.0 0.0 0.0 NHL_P 0.0 0.0 0.0 0.0 0.0 0.0 0.0 EMRGN 63.063.0 59.5 51.2 55.2 62.5 64.5 STD_N 63.0 63.0 59.5 51.2 55.2 62.5 64.5GRSNP 0.0 0.0 0.0 0.1 0.1 0.0 0.0 HSKCR 2.0 2.0 3.0 2.0 2.0 1.0 2.0HU5SN 1415.0 1329.5 1487.0 731.5 710.0 1383.0 1216.0 PLHTN 231.7 198.8199.2 234.2 251.2 209.5 182.4 ERHTN 97.3 75.7 80.3 92.6 92.2 79.8 73.6HU1PN 1250.0 1185.0 1422.0 671.0 617.5 1313.5 1127.5 HU9PN 1350.0 1280.51547.0 718.0 698.5 1392.0 1217.5 HUPSN 1301.0 1227.5 1473.5 691.5 671.01364.5 1171.0 PLQUR 4.5 6.5 5.0 7.0 9.0 5.5 8.0 EARPN 1.4 1.0 1.5 1.41.0 1.0 1.0 SHLNN 12.3 9.3 5.8 7.0 7.5 6.8 6.5 ALCPR 1.0 1.0 1.0 1.0 1.01.0 1.0 ALECN 18.0 18.0 18.0 18.0 18.0 18.0 18.0 ANTCN 5.0 6.0 8.5 5.05.0 5.0 5.0 COBCN 12.5 10.0 12.5 9.0 19.0 13.5 14.0 CR2LN 4.0 4.0 4.04.0 4.0 4.0 3.5 CRTYN 6.0 6.0 6.0 6.0 6.0 6.0 6.0 DA19N 4.0 5.0 5.0 2.03.5 4.0 4.5 DSAZN 59.5 55.5 62.5 30.5 30.0 58.0 51.0 DSGMN 46.5 49.055.0 58.0 43.0 53.5 52.0 GLCBN 1.0 1.0 1.0 1.0 1.0 1.0 1.0 GLCRN 3.0 2.02.0 2.0 1.0 3.0 1.0 HSKCN 2.5 2.5 2.0 3.0 2.0 2.5 2.5 HSKDN 21.0 21.021.0 21.0 21.0 21.0 21.0 HU19N 100.0 95.5 125.0 47.0 81.0 78.5 90.0HU25N 2433.0 2412.0 2636.5 2488.0 2360.0 2526.0 2368.0 HUSGN 1018.01082.5 1149.5 1025.0 940.0 1143.0 1152.0 KRCLN 8.0 8.0 8.0 8.0 . 8.0 8.0KRTEN 3.0 3.0 3.0 3.0 3.0 3.0 3.0 KRTPN 2.0 2.0 2.0 2.0 2.0 2.0 2.0SLKCN 3.0 3.0 3.0 5.0 5.0 3.0 1.0 LTEIN 15.0 11.0 10.5 14.0 14.0 13.012.0 ERLWN 8.7 8.7 8.6 9.4 9.2 9.0 8.6 ERLLN 72.2 78.3 71.8 93.4 79.272.3 66.9 LAERN 5.1 5.7 7.8 5.8 7.0 6.4 6.0 ANGBN 27.5 17.5 30.0 35.025.0 15.0 35.0 LTBRN 6.9 5.7 7.7 10.4 22.6 7.5 6.1 TBANN 35.0 30.0 42.550.0 50.0 17.5 20.0 LTASN 49.1 38.4 28.7 46.2 40.4 41.8 35.5 ERLNN 19.014.6 14.6 14.9 14.6 17.6 13.8 ERDIN 36.7 37.0 35.9 40.2 39.5 39.2 40.7EWGTN 103.1 80.6 64.5 96.6 92.0 123.7 95.4 KRRWN 11.4 15.0 12.4 15.615.6 12.6 16.4 KRLNN 10.5 10.0 11.3 9.5 11.5 9.8 9.3 KRWDN 8.5 7.0 8.37.5 7.5 8.0 7.0 KRDPN 5.0 4.0 4.3 5.5 5.0 5.8 4.0 KRPRN 51.5 55.5 43.075.0 62.0 63.5 31.0 COBDN 19.9 24.2 21.1 25.3 25.1 23.5 26.9 APBRR 2.04.0 3.5 2.0 2.0 2.5 2.0 LSPUR 2.0 8.5 2.5 1.0 4.0 4.0 7.0 MLWVR 5.0 5.53.5 5.0 7.0 3.0 4.0 LFLCR 5.0 4.5 4.5 2.0 3.0 2.5 4.0 HSKTR 3.0 4.5 8.07.0 6.0 4.5 2.0 HEPSR 1.5 2.0 1.0 1.0 2.0 1.0 3.0 KRRWR 2.0 2.0 2.0 2.02.0 2.0 2.0 ETAPR 1.5 1.0 1.0 1.0 1.0 1.0 1.5 K1KHN 31.5 24.5 29.0 28.027.0 31.0 23.0 TILLN 0.0 1.5 0.0 0.0 0.0 0.0 0.0 TILLP 0.0 2.4 0.0 0.00.0 0.0 0.0 DROPP 0.8 0.0 0.0 0.0 0.0 0.0 3.2 KERAR 1.5 1.0 1.0 1.0 1.01.5 1.0

The present invention has one of the best yields of all of thecomparison inbreds. The present invention shows a pollen shed rating of7.0 there are only 3 of the comparison inbreds with higher ratings. Therating scale of 9-1 is used for most rating data. In all instances 9 isthe best rating and 1 is the lowest rating. Heat Units per day werecalculated: HU=[MaxTemp (86)−Min Temp (50)]/2−50. An inbred's responseto environment is often more pronounced than a hybrid.

This is a summary of some of the color traits listed in Table 1 above.

SLKCN Silk color: 1.0 light green CR2LN Color of 2nd leaf above the ear4.0 very dark green (at anthesis): GLCRN Glume Color: 2.0 medium greenGLCBN Glume color bars perpendicular 1.0 absent to their veins (glumebands): 1. absent 2. present ANTCN Anther color: 6.0 pale yellow APBRRAnthocyanin pigment of brace 4.0 dark roots: 1. absent 2. faint 3.moderate 4. dark COBCN Cob color: 14.0 red KRCLN Hard endosperm color:8.0 yellow-orange ALECN Aleurone color: 18.0 colorless

The data provided above is often a color. The Munsell code is areference book of color, which is known and used in the industry and bypersons with ordinary skill in the art of plant breeding.

Hybrid Performance of G06-NP2899

Table 2 shows the inbred G06-NP2899 in hybrid combination in X72274 incomparison with a number of other hybrid combinations. The other hybridcombinations shown are commercial or experimental hybrids have a commoninbred with X72274. When in this hybrid combination the present inbredG06-NP2899 carries less moisture than Hybrid 2 but more moisture thanthe other hybrids. The present invention in this hybrid is not carryingthe yield that hybrids 1 and 2 are carrying. On the other hand X72274shows good agronomic performance with little tendency to stalk lodging.The test weight for the hybrid combination containing the presentinvention is heavier than all of the other hybrids except hybrid 1.

TABLE 2 PAIRED HYBRID COMPARISON DATA TW AbbrCode Yld Moist T PCTSLPCTRL PCTDE Stand Sgreen Gsnap Hybrid 1{circumflex over ( )} 211.2 17.856.7 0.6 12.1 0.0 61.4 19.3 0.0 Hybrid 2{circumflex over ( )} 207.1 19.455.6 1.0 14.7 0.0 63.1 20.1 0.0 X72274* 205.8 19.1 55.9 0.9 9.2 0.0 63.139.5 0.0 Hybrid 3{circumflex over ( )} 205.2 18.4 55.7 1.4 0.9 0.0 62.517.3 0.0 {circumflex over ( )}indicates a common inbred with X72274*indicates G06-NP2899 is in the hybrid

This invention also is directed to methods for producing a corn plant bycrossing a first parent corn plant with a second parent corn plantwherein the first or second parent corn plant is an inbred corn plantfrom the line G06-NP2899. Further, both first and second parent cornplants can come from the inbred corn line G06-NP2899 which produces aself of the inbred invention. The present invention can be employed in avariety of breeding methods which can be selected depending on the modeof reproduction, the trait, and the condition of the germplasm. Thus,any breeding methods using the inbred corn line G06-NP2899 are part ofthis invention: selfing, backcrosses, hybrid production, and crosses topopulations, and haploid by such old and known methods of using stocksix material that induces haploids and anther culturing and the like.

All plants and plant cells produced using inbred corn line G06-NP2899are within the scope of this invention. The invention encompasses theinbred corn line used in crosses with other, different, corn inbreds toproduce (F1) corn hybrid seeds and hybrid plants and the grain producedon the hybrid plant. This invention includes plant and plant cells,which upon growth and differentiation produce corn plants having thephysiological and morphological characteristics of the inbred lineG06-NP2899.

Additionally, this maize can, within the scope of the invention,contain: a mutant gene such as, but not limited to, the amylose,amylase, sugary 1 or shrunken 1 or waxy or AE or imazethapyr tolerant(IT or IR™) mutant gene; or transgenic genes such as but not limited toinsect resistant genes such as Corn Rootworm gene, Bacillusthuringiensis (Cry genes), or herbicide resistant genes such as Pat geneor Bar gene, EPSP, or disease resistant genes such as the Mosaic virusresistant gene, etc., or trait altering genes such as flowering genes,oil modifying genes, senescence genes and the like. The methods andtechniques for inserting, or producing and/or identifying a mutation ora transgene into the present invention through breeding, transformation,or mutating are well known and understood by those of ordinary skill inthe art.

A number of different inventions exist which are designed to avoiddetasseling in maize hybrid production. Some examples are switchablemale sterility, lethal genes in the pollen or anther, inducible malesterility, male sterility genes with chemical restorers, sterility geneslinked with parent. U.S. Pat. No. 6,025,546, relates to the use oftapetum-specific promoters and the barnase gene. U.S. Pat. No. 6,627,799relates to modifying stamen cells to provide male sterility. Therefore,one aspect of the current invention concerns the present inventioncomprising one or more gene(s) capable of restoring male fertility tomale-sterile maize inbreds or hybrids.

Various techniques for breeding and moving or altering genetic materialwithin or into the present invention (whether it is an inbred or inhybrid combination) are also known to those skilled in the art. Thesetechniques to list only a few are anther culturing, haploid production,(stock six is a method that has been in use for thirty years and is wellknown to those with skill in the art), transformation, irradiation toproduce mutations, chemical or biological mutation agents and a host ofother methods are within the scope of the invention. All parts of theG06-NP2899 plant including its plant cells produced using the inbredcorn line is within the scope of this invention. The term transgenicplant refers to plants having genetic sequences, which are introducedinto the genome of a plant by a transformation method and the progenythereof. Transformation methods are means for integrating new geneticcoding sequences into the plant's genome by the incorporation of thesesequences into a plant through man's assistance, but not by breedingpractices. The transgene once introduced into plant material andintegrated stably can be moved into other germplasm by standard breedingpractices.

Though there are a large number of known methods to transform plants,certain types of plants are more amenable to transformation than areothers. Transformation of dicots is usually achievable for example,tobacco is a readily transformable plant. Monocots can present sometransformation challenges, however, the basic steps of transformingplants monocots have been known in the art for about 15 years. The mostcommon method of maize transformation is referred to as gunning ormicroprojectile bombardment though other methods can be used. Theprocess employs small gold-coated particles coated with DNA which areshot into the transformable material. Detailed techniques for gunningDNA into cells, tissue, callus, embryos, and the like are well known inthe prior art. One example of steps that can be involved in monocottransformation are concisely outlined in U.S. Pat. No. 5,484,956“Fertile Transgenic Zea mays Plants Comprising Heterologous DNA EncodingBacillus Thuringiensis Endotoxin” issued Jan. 16, 1996 and also in U.S.Pat. No. 5,489,520 “Process of Producing Fertile Zea mays Plants andProgeny Comprising a Gene Encoding Phosphinothricin Acetyl Transferase”issued Feb. 6, 1996.

Plant cells such as maize can be transformed not only by the use of agunning device but also by a number of different techniques. Some ofthese techniques include maize pollen transformation (See University ofToledo 1993 U.S. Pat. No. 5,177,010); Whiskers technology (See U.S. Pat.Nos. 5,464,765 and 5,302,523); electroporation; PEG on Maize;Agrobacterium (See 1996 article on transformation of maize cells inNature Biotechnology, Volume 14, June 1996) along with numerous othermethods which may have slightly lower efficiency rates. Some of thesemethods require specific types of cells and other methods can bepracticed on any number of cell types.

The use of pollen, cotyledons, zygotic embryos, meristems and ovum asthe target issue can eliminate the need for extensive tissue culturework. Generally, cells derived from meristematic tissue are useful. Themethod of transformation of meristematic cells of cereal is taught inthe PCT application WO96/04392. Any number of various cell lines,tissues, calli and plant parts can and have been transformed by thosehaving knowledge in the art. Methods of preparing callus or protoplastsfrom various plants are well known in the art and specific methods aredetailed in patents and references used by those skilled in the art.Cultures can be initiated from most of the above-identified tissue. Theonly true requirement of the transforming plant material is that it canform a transformed plant.

The DNA used for transformation of these plants clearly may be circular,linear, and double or single stranded. Usually, the DNA is in the formof a plasmid. The plasmid usually contains regulatory and/or targetingsequences which assists the expression of the gene in the plant. Themethods of forming plasmids for transformation are known in the art.Plasmid components can include such items as: leader sequences, transitpolypeptides, promoters, terminators, genes, introns, marker genes, etc.The structures of the gene orientations can be sense, antisense, partialantisense, or partial sense: multiple gene copies can be used. Thetransgenic gene can come from various non-plant genes (such as;bacteria, yeast, animals, and viruses) along with being from plants.

The regulatory promoters employed can be constitutive such as CaMv35S(usually for dicots) and polyubiquitin for monocots or tissue specificpromoters such as CAB promoters, MR7 described in U.S. Pat. No.5,837,848, etc. The prior art promoters, includes but is not limited to,octopine synthase, nopaline synthase, CaMv19S, mannopine synthase. Theseregulatory sequences can be combined with introns, terminators,enhancers, leader sequences and the like in the material used fortransformation.

The isolated DNA is then transformed into the plant. After thetransformation of the plant material is complete, the next step isidentifying the cells or material, which has been transformed. In somecases, a screenable marker is employed such as the beta-glucuronidasegene of the uidA locus of E. coli. Then, the transformed cellsexpressing the colored protein are selected. In many cases, a selectablemarker identifies the transformed material. The putatively transformedmaterial is exposed to a toxic agent at varying concentrations. Thecells not transformed with the selectable marker, which providesresistance to this toxic agent, die. Cells or tissues containing theresistant selectable marker generally proliferate. It has been notedthat although selectable markers protect the cells from some of thetoxic affects of the herbicide or antibiotic, the cells may still beslightly affected by the toxic agent by having slower growth rates. Ifthe transformed material was cell lines then these lines are regeneratedinto plants. The cells' lines are treated to induce tissuedifferentiation. Methods of regeneration of cellular maize material arewell known in the art.

A deposit of at least 2500 seeds of this invention will be maintained bySyngenta Seed Inc. Access to this deposit will be available during thependency of this application to the Commissioner of Patents andTrademarks and persons determined by the Commissioner to be entitledthereto upon request. All restrictions on availability to the public ofsuch material will be removed upon issuance of a granted patent of thisapplication by depositing at least 2500 seeds of this invention at theAmerican Type Culture Collection (ATCC), at 10801 University Boulevard,Manassas, Va. 20110. The date of deposit was Apr. 18, 2008. The ATCCnumber of the deposit is PTA-9162 and on May 9, 2008 the seeds weretested and found to be viable. The deposit of at least 2500 seeds willbe from inbred seed taken from the deposit maintained by Syngenta SeedInc. The ATCC deposit will be maintained in that depository, which is apublic depository, for a period of 30 years, or 5 years after the lastrequest, or for the enforceable life of the patent, whichever is longer,and will be replaced if it becomes nonviable during that period.

Additional public information on patent variety protection may beavailable from the PVP Office, a division of the US Government.

Accordingly, the present invention has been described with some degreeof particularity directed to the preferred embodiment of the presentinvention. It should be appreciated, though that the present inventionis defined by the following claims construed in light of the prior artso that modifications or changes may be made to the preferred embodimentof the present invention without departing from the inventive conceptscontained herein.

1. Seed of maize inbred line designated G06-NP2899, representative seedof said line having been deposited under ATCC Accession No. PTA-9162. 2.A maize plant, or a part thereof, produced by growing the seed ofclaim
 1. 3. The maize plant of claim 2 wherein said plant has beendetasseled.
 4. A tissue culture of regenerable cells produced from theplant of claim
 2. 5. Protoplasts produced from the tissue culture ofclaim
 4. 6. The tissue culture of claim 4, wherein cells of the tissueculture are from a tissue selected from the group consisting of leaf,pollen, embryo, root, root tip, anther, silk, flower, kernel, ear, cob,husk and stalk.
 7. A maize plant regenerated from the tissue culture ofclaim 4, said plant having all the morphological and physiologicalcharacteristics of inbred line G06-NP2899, representative seed of saidline having been deposited under ATCC Accession No. PTA-9162.
 8. Amethod for producing an F1 hybrid maize seed, comprising crossing theplant of claim 2 with a different maize plant and harvesting theresultant F1 hybrid maize seed.
 9. A method of producing a male sterilemaize plant comprising transforming the maize plant of claim 2 with anucleic acid molecule that confers male sterility.
 10. A male sterilemaize plant produced by the method of claim
 9. 11. A method of producingan herbicide resistant maize plant comprising transforming the maizeplant of claim 2 with a transgene that confers herbicide resistance. 12.An herbicide resistant maize plant produced by the method of claim 11.13. The maize plant of claim 12, wherein the transgene confersresistance to an herbicide selected from the group consisting of:imidazolinone, sulfonylurea, glyphosate, glufosinate,L-phosphinothricin, triazine and benzonitrile.
 14. A method of producingan insect resistant maize plant comprising transforming the maize plantof claim 2 with a transgene that confers insect resistance.
 15. Aninsect resistant maize plant produced by the method of claim
 14. 16. Themaize plant of claim 15, wherein the transgene encodes a Bacillusthuringiensis endotoxin.
 17. A method of producing a disease resistantmaize plant comprising transforming the maize plant of claim 2 with atransgene that confers disease resistance.
 18. A disease resistant maizeplant produced by the method of claim
 17. 19. A method of producing amaize plant with decreased phytate content comprising transforming themaize plant of claim 2 with a transgene encoding phytase.
 20. A maizeplant with decreased phytate content produced by the method of claim 19.21. A method of producing a maize plant with modified fatty acidmetabolism or modified carbohydrate metabolism comprising transformingthe maize plant of claim 2 with a transgene encoding a protein selectedfrom the group consisting of stearyl-ACP desaturase,fructosyltransferase, levansucrase, alpha-amylase, invertase and starchbranching enzyme.
 22. A maize plant produced by the method of claim 21.23. The maize plant of claim 22 wherein the transgene confers a traitselected from the group consisting of waxy starch and increased amylosestarch.
 24. A maize plant, or part thereof, having all the physiologicaland morphological characteristics of the inbred line G06-NP2899,representative seed of said line having been deposited under ATCCAccession No. PTA-9162.
 25. A method of introducing a desired trait intomaize inbred line G06-NP2899 comprising: (a) crossing G06-NP2899 plantsgrown from G06-NP2899 seed, representative seed of which has beendeposited under ATCC Accession No. PTA-9162, with plants of anothermaize line that comprise a desired trait to produce F1 progeny plants,wherein the desired trait is selected from the group consisting of malesterility, herbicide resistance, insect resistance, disease resistanceand waxy starch; (b) selecting F1 progeny plants that have the desiredtrait to produce selected F 1 progeny plants; (c) crossing the selectedprogeny plants with the G06-NP2899 plants to produce backcross progenyplants; (d) selecting for backcross progeny plants that have the desiredtrait and physiological and morphological characteristics of maizeinbred line G06-NP2899 listed in Table 1 to produce selected backcrossprogeny plants; and (e) repeating steps (c) and (d) three or more timesin succession to produce selected fourth or higher backcross progenyplants that comprise the desired trait and all of the physiological andmorphological characteristics of maize inbred line G06-NP2899 listed inTable 1 as determined at the 5% significance level when grown in thesame environmental conditions.
 26. A plant produced by the method ofclaim 25, wherein the plant has the desired trait and all of thephysiological and morphological characteristics of maize inbred lineG06-NP2899 listed in Table 1 as determined at the 5% significance levelwhen grown in the same environmental conditions.
 27. The plant of claim26 wherein the desired trait is herbicide resistance and the resistanceis conferred to an herbicide selected from the group consisting of:imidazolinone, sulfonylurea, glyphosate, glufosinate,L-phosphinothricin, triazine and benzonitrile.
 28. The plant of claim 26wherein the desired trait is insect resistance and the insect resistanceis conferred by a transgene encoding a Bacillus thuringiensis endotoxin.29. The plant of claim 26 wherein the desired trait is male sterilityand the trait is conferred by a cytoplasmic nucleic acid molecule thatconfers male sterility.
 30. A method of modifying fatty acid metabolism,modified phytic acid metabolism or modified carbohydrate metabolism intomaize inbred line G06-NP2899 comprising: (a) crossing G06-NP2899 plantsgrown from G06-NP2899 seed, representative seed of which has beendeposited under ATCC Accession No. PTA-9162, with plants of anothermaize line that comprise a nucleic acid molecule encoding an enzymeselected from the group consisting of phytase, stearyl-ACP desaturase,fructosyltransferase, levansucrase, alphaamylase, invertase and starchbranching enzyme; (b) selecting F1 progeny plants that have said nucleicacid molecule to produce selected F 1 progeny plants; (c) crossing theselected progeny plants with the G06-NP2899 plants to produce backcrossprogeny plants; (d) selecting for backcross progeny plants that havesaid nucleic acid molecule and physiological and morphologicalcharacteristics of maize inbred line G06-NP2899 listed in Table 1 toproduce selected backcross progeny plants; and (e) repeating steps (c)and (d) three or more times in succession to produce selected fourth orhigher backcross progeny plants that comprise said nucleic acid moleculeand have all of the physiological and morphological characteristics ofmaize inbred line G06-NP2899 listed in Table 1 as determined at the 5%significance level when grown in the same environmental conditions. 31.A plant produced by the method of claim 30, wherein the plant comprisesthe nucleic acid molecule and has all of the physiological andmorphological characteristics of maize inbred line G06-NP2899 listed inTable 1 as determined at the 5% significance level when grown in thesame environmental conditions.